青霉素G(BP)ELISA快速检测试剂盒
一、原理
本试剂盒采用间接竞争ELISA方法，在微孔条上预包被偶联抗原，样本中的残留物青霉素将和微孔条上预包被的偶联抗原竞争抗青霉素抗体，加入酶标记物后，用TMB底物显色，样本吸光值与其所含残留物青霉素的含量成负相关，与标准曲线比较即可得出相应残留物青霉素的含量。
二、试剂盒特性
u 试剂盒灵敏度： 0.1ppb
u 孵育温度： 25℃
u 孵育时间： 30min～30min～15min
u 批内、批间差： 不超过10%
u 样本检测下限
组织 ················································ 2ppb
u 交叉反应率
青霉素· ········································ 100%
氨苄青霉素········································ 0.8%
邻氯青霉素 ······································· 0.2%
双氯青霉素 ······································· 0.1%
阿莫西林 ········································· 0.1%
头孢塞呋 ········································· 0.1%
u 样本回收率
组 织 ········································· 60%-120%
三、试剂盒组成
1 | 微量测试孔 | 每条8孔，一板12条 | |
2 | 标准液×6瓶 （1ml/瓶） | 0ppb | 0.1ppb |
0.3ppb | 0.9ppb | ||
2.7ppb | 8.1ppb | ||
3 | 酶标记物 | 12ml | 红色帽 |
4 | 抗体工作液 | 7ml | 蓝色帽 |
5 | 底物A液 | 7ml | 白色帽 |
6 | 底物B液 | 7ml | 黑色帽 |
7 | 终止液 | 7ml | 黄色帽 |
8 | 20X浓缩洗涤液 | 40ml | 白色帽 |
9 | 10X浓缩复溶液 | 50ml | 透明帽 |
四、样品前处理步骤及检测分析步骤
详见产品说明书。
五、储藏条件和保质期
1、 储藏条件：试剂盒于2-8℃保存，不要冷冻。
2、 保 质 期：该产品有效期为1年，生产日期见包装盒。
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